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Literature summary extracted from

  • Fernandez, I.S.; Staendker, L.; Maegert, H.J.; Forssmann, W.G.; Gimenez-Gallego, G.; Romero, A.
    Crystal structure of human epidermal kallikrein 7 (hK7) synthesized directly in its native state in E. coli: insights into the atomic basis of its inhibition by LEKTI domain 6 (LD6) (2008), J. Mol. Biol., 377, 1488-1497.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.117 gene KLK7, expression in Escherichia coli with secretion to the the bacterial periplasm Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.117 purified recombinant enzyme bound to specific inhibitor LEKTI domain 6, 8 mg/ml protein in sodium phosphate buffer, pH 7.5, with a reservoir solution containing 3.2 M ammonium sulfate in 0.1 M HEPES, pH 7.0, X-ray diffraction structure determination and anaylsis at 2.8 A resolution Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.117 LEKTI domain 6 i.e. lympho-epithelial Kazaltype-related inhibitor domain 6, specific inhibition, interaction and binding structure analysis, overview Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.117 0.0159
-
 Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.117 Homo sapiens P49862 gene KLK7
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.117 brain
-
 Homo sapiens
-
3.4.21.117 epidermis
-
 Homo sapiens
-
3.4.21.117 lung adenocarcinoma cell
-
 Homo sapiens
-
3.4.21.117 lymphoblastic leukemia cell
-
 Homo sapiens
-
3.4.21.117 ovary
-
 Homo sapiens
-
3.4.21.117 pancreatic cancer cell
-
 Homo sapiens
-
3.4.21.117 skin kallikrein 7 is the major protease in human skin Homo sapiens
-
3.4.21.117 testicular carcinoma cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.117 Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 + H2O
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.117 More three-dimensional structure and specific substrate site, involving conserved residues Ala190-Ser195, Val213-Cys220 and Pro225-Tyr228, molecular modeling, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.21.117 kallikrein 7
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.117 37
-
 assay at Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.21.117 0.026
-
-
 Homo sapiens LEKTI domain 6